Studies on Pectic Enzymes of Microorganisms Part II

Pectic Enzymes

Indoleacetic acid (IAA) and/or inhibitors of DNA, RNA or protein synthesis were added to the apex of decapitated seedlings of Pisum sativum L. var. Alaska. At various times up to 4 days, enzymic protein was extraoted from a segment of epicotyl immediately below the apex and assayed for its ability to hydrolyse polysaccharides or their derivatives. With the exception of am;ylase, the total amoun...

Relationship of Pectic Enzymes to Abscission

A comprehensive review of work pertinent to changes in the separation zone prior to abscission is presented. Since these changes are largely pectic in nature the involvement of pectic enzymes is suggested. The results obtained for the role of pectin methylesterase ( PME) in abscission arc as follows: ( 1 ) A localization of PME activity in the abscission zone of tobacco pedicels was established...

Production of pectic enzymes in yeasts.

When grown in the appropriate medium, several yeast species produce pectinases able to degrade pectic substances. It is mainly exocellular endopolygalacturonases that break pectins or pectate down by hydrolysis of alpha-1,4-glycosidic linkages in a random way. Biochemical characterisation of these enzymes has shown that they have an optimal pH in the acidic region and an optimal temperature bet...

Part II Ancient Microorganisms Bacteria Chapter 6 Palaeomicrobiology of Tuberculosis

The study of tuberculosis palaeomicrobiology has proved to be most rewarding. Due to the characteristic palaeopathological lesions, tuberculosis was recognised in archaeological material and was the first infectious disease to be studied by modern biomolecular methods. The combination of a tough bacterial cell wall and GC-rich DNA has resulted in excellent DNA preservation in some specimens. A ...

Studies on histamine-metabolizing enzymes in intact animals. II.